Comparison of Three Sperm-Counting Methods for the Determination of Sperm Concentration in Human Semen and Sperm Suspensions

The Influence of Sperm Morphology, Total Motile Sperm Count of Semen and the Number of Motile Sperm Inseminated in Sperm Samples on the Success of Intrauterine Insemination

Accuracy Evaluation of The Depth of Six Kinds of Sperm Counting Chambers for both Manual and Computer-Aided Semen Analyses

Background Although the depth of the counting chamber is an important factor influencing sperm counting, no research has yet been reported on the measurement and comparison of the depth of the chamber. We measured the exact depths of six kinds of sperm counting chambers and evaluated their accuracy. MaterialsAndMethods In this prospective study, the depths of six kinds of sperm counting chamber...

P-19: Effect of Three Sperm Preparation Methods on Sperm DNA Integrity and Oocyte Fertilization Rate

Background: Techniques of sperm preparation require centrifugation to separate spermatozoa from the seminal plasma. The centrifugation can induce damage to the spermatozoa and increase reactive oxygen species(ROS) productionspecially in leukospermic speciemens that are associated with impaired function and fertilizing capacity. However in ICSI, fertilization can occure with damage DNA sperm but...

Quality Comparison of Three Disposable Sperm Cell Counting Chambers

AND CONCLUSION In this study various quality aspects of the CellVision, Leja, and MicroCell disposable sperm cell counting slides were investigated. The height of the chambers was determined using interferometric spectrometry. The average heights of the Leja and MicroCell were very close, respectively 20.0 μm and 20.3 μm, whereas the CellVision was lower than specification (17.9 μm). The accura...

Validation of the sperm class analyser CASA system for sperm counting in a busy diagnostic semen analysis laboratory.

Sperm counts have been linked to several fertility outcomes making them an essential parameter of semen analysis. It has become increasingly recognised that Computer-Assisted Semen Analysis (CASA) provides improved precision over manual methods but that systems are seldom validated robustly for use. The objective of this study was to gather the evidence to validate or reject the Sperm Class Ana...